Antimicrobial Metabolite from the Culture of Endophytic Fungus AFK-8 Isolated from Kayu Kuning [Archangelisia flava (L.) Merr.]

The fungus AFK-8 is one of endophytic filamentous fungus isolated from young stems of kayu kuning [Archangelesia flava (L.) Merr.]. The ethyl acetate extract derived from fungus culture shows their antibiotic activity tested against bacteria and fungi on a disc diffusion method. Scaling up cultivation of endophytic fungus AFK-8 on 1L PDA for 3 weeks yielded 143.3 mg of chloroform extract. Fractionation guided assay of these extract showed that the fraction 6 is the most active fraction against tested microbes with MIC values 8, 32, 4 and 64 􀀀g/ml against B. subtilis, M. luteus, S. aureus dan E. coli respectively. This fraction is also active against C. albicans, R. minuta and A. niger with MIC value 32 mg/ml. The proposed chemical structure of active metabolit in fraction 6 is 1,2-diamino-9,10-anthracenedione which was determined through a GCMS analysis. International Seminar Biotechnology. Hal. 1-9